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Journal: Antimicrobial Agents and Chemotherapy
Article Title: Piperidine-4-Carboxamides Target DNA Gyrase in Mycobacterium abscessus
doi: 10.1128/AAC.00676-21
Figure Lengend Snippet: Bactericidal and antibiofilm activity of 844 and 844-TFM against M. abscessus . (A) Exponentially growing suspension cultures of M. abscessus subsp. abscessus ATCC 19977 were treated with 1×, 4×, and 8× MIC of 844 or 844-TFM, moxifloxacin (MXF), SPR719, or clarithromycin (CLR), and CFU were enumerated by plating samples on agar after 2 and 3 days. (B) Exponentially growing biofilm cultures were treated with 1×, 4×, and 8× MIC of 844, 844-TFM, MXF, SPR719, or CLR, and surface-attached CFU were enumerated by suspending bacteria and plating on agar after 2 and 3 days. MXF, SPR719, and CLR are included for comparison (MXF, SPR719) or as control (CLR). Experiments in panels A and B were carried out three times independently, and the results are represented as mean values with the standard deviations displayed as error bars. A two-way ANOVA multiple comparison test was performed using GraphPad Prism 8 software to compare treated groups with untreated day 0 CFU.
Article Snippet: The antibiofilm activity of 844 and 844-TMF was determined employing the MBEC 96-well
Techniques: Activity Assay, Suspension, Bacteria, Comparison, Control, Software